Isolation and characterization of the human hepatic lipase gene.

Functional characterization of a chimeric lipase genetically engineered from human lipoprotein lipase and human hepatic lipase.

Lipoprotein lipase (LPL) and hepatic lipase (HL) mediate the hydrolysis of triglycerides and phospholipids present in circulating lipoprotein particles and are essential for normal lipid metabolism. Both enzymes have a similar primary amino acid structure and share requirements for intact catalytic, lipid binding, and heparin binding domains. However, LPL and HL exhibit different substrate spec...

Transcriptional regulation of the human hepatic lipase (LIPC) gene promoter.

Hepatic lipase (HL) plays a key role in the metabolism of plasma lipoproteins, and its level of activity requires tight regulation, given the association of both low and high levels with atherosclerosis and coronary artery disease. However, little is known about the factors responsible for HL expression. Here, we report that the human hepatic lipase gene (LIPC) promoter is regulated by hepatocy...

Two polymorphisms for the human hepatic lipase (HL) gene.

SOURCE AND DESCRIPTION OF CLONE: A 1.65 kb ECORI cDNA insert corresponding to the full length hepatic lipase (HL) sequence isolated from a rat liver cDNA library (I). CHROMOSOMAL LOCALIZATION: Chromosome 15q21 by somatic cell and in situ hybridization (2).

Isolation and characterization of a staphylococcal lipase.

A number of coagulase-negative staphylococci isolated from human skin were found to produce lipase. Lipolytic activity appeared in the growth medium during the stationary phase of growth but did not appear as a result of autolysis of the cells. Maximal lipase synthesis was obtained when the medium was adjusted to pH 7.5 before inoculation. The purified enzyme hydrolyzed tributyrin and tridecano...

isolation and characterization of thermophilic and psychrophilic lipase-producing organisms